1,25-Dihydroxyvitamin D3-induced calcium-binding protein: localization in organ-cultured embryonic chick duodenum. | Zendy

R.A. Corradino | Zendy; A N Taylor | Zendy

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1,25-Dihydroxyvitamin D3-induced calcium-binding protein: localization in organ-cultured embryonic chick duodenum.

Author(s) -

R.A. Corradino,

A N Taylor

Publication year - 1985

Publication title -

journal of histochemistry and cytochemistry

Language(s) - Uncategorized

Resource type - Journals

SCImago Journal Rank - 0.971

H-Index - 124

eISSN - 1551-5044

pISSN - 0022-1554

DOI - 10.1177/33.5.2580884

Subject(s) - organ culture , duodenum , calcium , calcium binding protein , embryonic stem cell , vitamin d dependent calcium binding protein , cytoplasm , microbiology and biotechnology , biology , in vivo , medicine , endocrinology , chemistry , in vitro , biochemistry , gene

1,25-Dihydroxyvitamin D3 (1,25(OH)2D3) induces de novo biosynthesis of a specific calcium-binding protein (CaBP) in embryonic chick duodenum in organ culture. Using a highly sensitive and specific, peroxidase-antiperoxidase immunocytochemical procedure, 1,25(OH)2D3-induced CaBP in the organ-cultured duodenum was found only in the cytoplasm of absorptive cells, corresponding to its localization in rachitic chick duodenal cells after a single injection of 1,25(OH)2D3 in vivo. This observation, along with evidence correlating CaBP with calcium transport, strongly supports the use of the embryonic chick duodenal organ culture system as a physiologically relevant model of the vitamin D-dependent calcium absorptive mechanism.

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