An economical minichamber for immunohistochemical incubation. | Zendy

Mary Faith Abbuhl | Zendy; Manuel E. Velasco | Zendy

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An economical minichamber for immunohistochemical incubation.

Author(s) -

Mary Faith Abbuhl,

Manuel E. Velasco

Publication year - 1985

Publication title -

journal of histochemistry and cytochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.971

H-Index - 124

eISSN - 1551-5044

pISSN - 0022-1554

DOI - 10.1177/33.2.2578500

Subject(s) - antiserum , incubation , staining , immunohistochemistry , incubation period , chemistry , biology , pathology , antibody , biochemistry , immunology , medicine

A simple and economical "slide-minichamber" method for incubating tissue sections with antisera in immunohistochemical (peroxidase-antiperoxidase) staining procedures is described. The technique requires only materials routinely used in the laboratory. The method permits prolonged incubation of tissue sections with antiserum at 4 degrees C or at room temperature, use of small quantities of antiserum, and simultaneous incubation of two tissue sections with the same small quantity of antiserum, thereby allowing use of very dilute antisera and conservation of antisera when availability is limited.

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