Radioimmunocytochemical localization of retinal S-antigen with monoclonal antibodies. | Zendy

Noveen D. Das | Zendy; Robert J. Ulshafer | Zendy; Z. Suzanne Zam | Zendy; Victor R. Leverenz | Zendy; Hitoshi Shichi | Zendy

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Radioimmunocytochemical localization of retinal S-antigen with monoclonal antibodies.

Author(s) -

Noveen D. Das,

Robert J. Ulshafer,

Z. Suzanne Zam,

Victor R. Leverenz,

Hitoshi Shichi

Publication year - 1984

Publication title -

journal of histochemistry and cytochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.971

H-Index - 124

eISSN - 1551-5044

pISSN - 0022-1554

DOI - 10.1177/32.8.6379036

Subject(s) - antigen , monoclonal antibody , antibody , microbiology and biotechnology , biology , retinal , chemistry , immunology , biochemistry

Two monoclonal antibodies (RSA1/83 and RSA2/83) were developed against a homogeneous preparation of bovine retinal S-antigen. The two hybridomas produced by mouse X mouse hybrid myeloma cells secrete immunoglobulin G. Indirect autoradiography on glutaraldehyde-fixed preparations of bovine explants was used to locate the antigenic site. Antibody RSA1/83 recognizes the antigen primarily in the apical region of the rod outer segment, while antibody RSA2/83 located the antigen both in the outer and inner segments of the rod photoreceptor cells. A distinct band of silver grains also appeared along the inner limiting membrane with both antibodies. Control explants showed no specific labeling pattern over the various retinal compartments.

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