Fractionation and further characterization of granulocytic and monocytic alpha-naphthyl acetate (ANAE) esterases. | Zendy

Colin Scott | Zendy; D. Hough | Zendy; A. G. Bynoe | Zendy; D. B. Jones | Zendy; B. E. Roberts | Zendy

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Fractionation and further characterization of granulocytic and monocytic alpha-naphthyl acetate (ANAE) esterases.

Author(s) -

Colin Scott,

D. Hough,

A. G. Bynoe,

D. B. Jones,

B. E. Roberts

Publication year - 1984

Publication title -

journal of histochemistry and cytochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.971

H-Index - 124

eISSN - 1551-5044

pISSN - 0022-1554

DOI - 10.1177/32.6.6725934

Subject(s) - isoelectric focusing , isoelectric point , esterase , enzyme , isozyme , chemistry , biochemistry , pi , microbiology and biotechnology , fractionation , cytoplasm , sodium fluoride , chromatography , biology , fluoride , inorganic chemistry

Following characterization of myeloid nonspecific esterases by isoelectric focusing (IEF), two main groups of alpha-naphthyl acetate (ANAE) esterase isoenzymes were defined and fractionated from cytoplasmic extracts by chromato focusing techniques according to differences in their isoelectric points (pI). The first of these ANAE enzyme groups was common to leukocytes of both granulocytic and monocytic lineage, while the other, which characteristically comprised a group of isoenzymes within the pI range 5.5-6.1, was specifically associated with monocytic differentiation. The properties of the two purified ANAE enzyme fractions were compared by inhibition (heat and sodium fluoride) and further electrophoretic studies, and the results discussed in relation to the cytochemical characterization of these enzymes as markers of specific myeloid cell differentiation.

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