Visualization of peptide-immunoreactive processes on serotonin-immunoreactive cells using two-color immunoperoxidase staining. | Zendy

M.B. Hancock | Zendy

Open Access

Visualization of peptide-immunoreactive processes on serotonin-immunoreactive cells using two-color immunoperoxidase staining.

Author(s) -

M.B. Hancock

Publication year - 1984

Publication title -

journal of histochemistry and cytochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.971

H-Index - 124

eISSN - 1551-5044

pISSN - 0022-1554

DOI - 10.1177/32.3.6198359

Subject(s) - serotonin , immunoperoxidase , medulla , chemistry , medulla oblongata , hamster , immunocytochemistry , enkephalin , raphe nuclei , endocrinology , medicine , biology , anatomy , central nervous system , antibody , biochemistry , serotonergic , receptor , immunology , opioid , monoclonal antibody

The sequential application of the peroxidase-antiperoxidase (PAP) technique with nickel-intensified DAB and with DAB alone was used to visualize black peptide-immunoreactive endings on amber serotonin-immunoreactive cells in 1-2-micron paraffin sections of the hamster medulla. Met-enkephalin- and substance P-positive terminals were present on serotonin cells in the raphe nuclei, the ventral reticular formation, and in the nucleus interfascicularis hypoglossi. The presence of enkephalin-immunoreactive endings on medullary serotonin-immunoreactive cells correlates with the analgesia and autonomic changes that result from the application of morphine or met-enkephalin to the medulla.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research