Immunocytochemical localization of Na+,K+ adenosine triphosphatase in the rat retina.
Author(s) -
W L Stahl,
D G Baskin
Publication year - 1984
Publication title -
journal of histochemistry and cytochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.971
H-Index - 124
eISSN - 1551-5044
pISSN - 0022-1554
DOI - 10.1177/32.2.6319483
Subject(s) - retina , immunostaining , inner plexiform layer , atpase , adenosine triphosphatase , polyclonal antibodies , immunocytochemistry , triphosphatase , biology , chemistry , microbiology and biotechnology , biophysics , enzyme , biochemistry , immunohistochemistry , antibody , neuroscience , endocrinology , immunology
Immunocytochemical localization of Na+,K+-adenosine triphosphatase (ATPase) in fixed sections of rat retina was carried out using polyclonal antibodies to the highly purified enzyme and peroxidase-antiperoxidase and immunofluorescence techniques. In the photoreceptor layer of the rat retina, Na+,K+-ATPase immunoreactive sites were associated primarily with inner segments and only background activity was associated with the outer segments. This localization supports the proposed distribution of Na+,K+-ATPase in photoreceptor cells suggested by physiological studies. The Na+,K+-ATPase immunoreactive sites were also localized in the inner and outer plexiform layers, which are rich in synaptic contacts. The reticular pattern of immunostaining in those regions suggests that Na+,K+-ATPase immunoreactivity is associated with neuronal processes in those layers.
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