Open AccessGrowth hormone releasing factor (GRF) immunoreactivity in human and rat gastrointestinal tract and pancreas.
Author(s) -
Fred T. Bosman,
Chris van Assche,
A C Nieuwenhuyzen Kruseman,
Sue Jackson,
Philip J. Lowry
Publication year - 1984
Publication title -
journal of histochemistry and cytochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.971
H-Index - 124
eISSN - 1551-5044
pISSN - 0022-1554
DOI - 10.1177/32.11.6436365
Subject(s) - gastrin , pancreatic polypeptide , antrum , medicine , endocrinology , antiserum , enteroendocrine cell , pancreas , immunoperoxidase , endocrine system , staining , biology , hypothalamus , pancreatic islets , gastrointestinal tract , islet , hormone , glucagon , stomach , antibody , secretion , insulin , immunology , genetics , monoclonal antibody
Rabbit antisera were raised against a synthetic growth hormone releasing factor, which was originally isolated from a human pancreatic endocrine tumor (hpGRF-44). The antisera obtained showed no significant cross-reactivity with a variety of neurohormonal peptides. In addition to its occurrence in the human, but not in the rat, hypothalamus, hpGRF-44-like immunoreactivity was identified in human gastric antrum and human as well as rat pancreatic islets, using an indirect immunoperoxidase technique. Staining of serial sections and double staining revealed that in the gastric antrum the immunoreactivity was largely confined to gastrin (G) cells, whereas in pancreatic islets polypeptide (pp) cells were reactive. The physiological significance of these findings remains to be established.
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