Open AccessImmunocytochemical study of collagen in epidermal growth factor (EGF)-treated osteoblastic cells.
Author(s) -
Yasuyoshi Osaki,
Mayuri Tsunoi,
Y Hakeda,
Kojiro Kurisu,
Masayoshi Kumegawa
Publication year - 1984
Publication title -
journal of histochemistry and cytochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.971
H-Index - 124
eISSN - 1551-5044
pISSN - 0022-1554
DOI - 10.1177/32.11.6386976
Subject(s) - epidermal growth factor , clone (java method) , collagen, type i, alpha 1 , type i collagen , chemistry , endocrinology , type iv collagen , growth factor , microbiology and biotechnology , medicine , biology , extracellular matrix , receptor , biochemistry , gene , laminin
The alteration of collagen components in clone MC3T3-E1 cells by epidermal growth factor (EGF) was investigated immunocytochemically, using antibodies to type I and type III collagens. EGF transformed those cells that had become more slender than those of control cultures. Type I and type III collagens were observed in the same cells in both EGF-treated and control cultures. Type I collagen was decreased by EGF, whereas type III collagen appeared to be increased. However, no cells with only type III collagen were observed, suggesting that EGF influences collagen metabolism in clone MC3T3-E1 cells.
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