Visualization of intracellular trafficking: use of biotinylated ligands in conjunction with avidin-gold colloids. | Zendy

Randal E. Morris | Zendy; Catharine B. Saelinger | Zendy

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Visualization of intracellular trafficking: use of biotinylated ligands in conjunction with avidin-gold colloids.

Author(s) -

Randal E. Morris,

Catharine B. Saelinger

Publication year - 1984

Publication title -

journal of histochemistry and cytochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.971

H-Index - 124

eISSN - 1551-5044

pISSN - 0022-1554

DOI - 10.1177/32.1.6690597

Subject(s) - biotinylation , internalization , avidin , intracellular , biophysics , chemistry , ligand (biochemistry) , membrane , cell membrane , microbiology and biotechnology , cell , nanotechnology , biochemistry , materials science , biology , receptor

A simple, sensitive method to visualize the binding and internalization of protein ligands by cells in culture is described. A biotinylated toxin was used as ligand, and succinoylated avidin adsorbed onto 5.2 nm gold sols was the electron-dense marker. This method affords direct localization of proteins that are on the cell surface or intracellular without need for techniques that alter membrane integrity.

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