Immunohistochemical demonstration of keratins in human ovarian neoplasms. A comparison of methods. | Zendy

Raymond B. Nagle | Zendy; Virginia A. Clark | Zendy; Kathleen McDaniel | Zendy; J.R. Davis | Zendy

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Immunohistochemical demonstration of keratins in human ovarian neoplasms. A comparison of methods.

Author(s) -

Raymond B. Nagle,

Virginia A. Clark,

Kathleen McDaniel,

J.R. Davis

Publication year - 1983

Publication title -

journal of histochemistry and cytochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.971

H-Index - 124

eISSN - 1551-5044

pISSN - 0022-1554

DOI - 10.1177/31.8.6190854

Subject(s) - peroxidase , keratin , immunohistochemistry , polyclonal antibodies , pathology , dysgerminoma , primary and secondary antibodies , stain , ovary , chemistry , antibody , biology , staining , microbiology and biotechnology , medicine , biochemistry , enzyme , immunology , endocrinology

A comparison of five immunohistochemical methods for the demonstration of keratins in human ovarian neoplasms using affinity-purified polyclonal rabbit antibody was made. The use of indirect immunofluorescence on frozen sections briefly fixed in acetone was found to be the most sensitive method and demonstrated keratin in all 14 primary and 1 metastatic ovarian epithelial neoplasms studied. Protein A-peroxidase, peroxidase--antiperoxidase (PAP), indirect peroxidase, or the avidin--biotinylated peroxidase complex (ABC) methods applied to formalin-fixed tissues were less sensitive and led to false negative results in 9 of 15, 1 of 15, 8 of 15, and 6 of 15 cases, respectively. A single case of dysgerminoma failed to reveal keratin by any method.

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