Preparation of representative samples of subcellular fractions for electron microscopy by filtration with dextran. | Zendy

P. Palade | Zendy; A Saito | Zendy; Ryan Mitchell | Zendy; Sarah Fleischer | Zendy

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Preparation of representative samples of subcellular fractions for electron microscopy by filtration with dextran.

Author(s) -

P. Palade,

A Saito,

Ryan Mitchell,

Sarah Fleischer

Publication year - 1983

Publication title -

journal of histochemistry and cytochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.971

H-Index - 124

eISSN - 1551-5044

pISSN - 0022-1554

DOI - 10.1177/31.7.6189886

Subject(s) - filtration (mathematics) , dextran , chromatography , electron microscope , size exclusion chromatography , chemistry , polymer , materials science , transmission electron microscopy , microscopy , sample (material) , filter (signal processing) , scanning electron microscope , filter paper , analytical chemistry (journal) , nanotechnology , composite material , optics , mathematics , computer science , physics , biochemistry , statistics , enzyme , computer vision

A simple method is described for obtaining a representative field of subcellular fractions for thin section electron microscopy. The prefixed sample is admixed together with high molecular weight dextran, filtered onto a Millipore filter, and embedded in polymer. Filtration serves to uniformly distribute structures of different sizes and densities. The dextran serves as a nonosmophilic spacer, increasing the space between individual structures and preventing sample compression during filtration onto Millipore filters. Sample aggregation can also readily be assessed by the procedure.

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