A new technique for staining sections and recovering celloidin film in high resolution autoradiography.
Author(s) -
G Pradal,
P. Barrière,
F Mollat,
MariePaule Lefranc
Publication year - 1983
Publication title -
journal of histochemistry and cytochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.971
H-Index - 124
eISSN - 1551-5044
pISSN - 0022-1554
DOI - 10.1177/31.7.6189885
Subject(s) - staining , filter paper , materials science , high resolution , chemistry , pathology , geology , chromatography , medicine , remote sensing
Ultrathin sections are stained immediately after cutting by placing them in contact with staining solution and then placed on a slide covered by a celloidin film. This method largely avoids precipitates of heavy metals. The recovering of celloidin film is improved using a stainless steel basket. This technique is far more reliable than that involving use of a filter paper.
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