Rapid method for identification of macrophages in suspension by acid alpha-naphthyl acetate esterase activity. | Zendy

David L. Ennist | Zendy; Kerina Jones | Zendy

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Rapid method for identification of macrophages in suspension by acid alpha-naphthyl acetate esterase activity.

Author(s) -

David L. Ennist,

Kerina Jones

Publication year - 1983

Publication title -

journal of histochemistry and cytochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.971

H-Index - 124

eISSN - 1551-5044

pISSN - 0022-1554

DOI - 10.1177/31.7.6189884

Subject(s) - staining , esterase , phagocytosis , stain , chemistry , incubation , substrate (aquarium) , macrophage , microbiology and biotechnology , suspension culture , biochemistry , chromatography , cell culture , enzyme , in vitro , biology , ecology , genetics

A supravital staining procedure for the identification of macrophages in cell suspension using a modification of a standard cytochemical assay for alpha-naphthyl acetate esterase (ANAE) activity is described. Macrophages are stained an intense red-brown after 5 min incubation in a buffer using ANAE as the substrate and hexazonium pararosaniline as the coupler for the azo dye. There is close agreement in the number of ANAE-positive cells found and the number of macrophages identified in smears by morphological criteria, by phagocytosis, and by the presence of Fc receptors. Therefore, this stain provides a quick, inexpensive method to estimate the number of macrophages present in suspensions of lymphocytic tissues from rats and mice.

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