Immunogold-silver staining: new method of immunostaining with enhanced sensitivity. | Zendy

C S Holgate | Zendy; Peter K. Jackson | Zendy; Philip J. Cowen | Zendy; C C Bird | Zendy

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Immunogold-silver staining: new method of immunostaining with enhanced sensitivity.

Author(s) -

C S Holgate,

Peter K. Jackson,

Philip J. Cowen,

C C Bird

Publication year - 1983

Publication title -

journal of histochemistry and cytochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.971

H-Index - 124

eISSN - 1551-5044

pISSN - 0022-1554

DOI - 10.1177/31.7.6189883

Subject(s) - immunogold labelling , immunolabeling , immunostaining , immunoperoxidase , staining , antiserum , colloidal gold , antigen , chemistry , immunocytochemistry , tonsil , primary and secondary antibodies , silver stain , antibody , microbiology and biotechnology , pathology , biology , immunohistochemistry , materials science , immunology , nanotechnology , medicine , monoclonal antibody , nanoparticle

A new method for demonstrating antigens in paraffin sections of formol sublimate-fixed tissue is described that utilizes an "indirect" immunohistological technique employing immunoglobulin adsorbed to colloidal gold as the secondary antiserum. The gold particles introduced to antigenic sites are revealed by a silver precipitation reaction. This technique, the immunogold-silver staining method, is of much enhanced sensitivity (up to 200-fold) as compared with standard immunoperoxidase and immunogold staining methods. The results have been confirmed in a study of immunoglobulins in reactive human tonsil. The use of this new method for double immunolabeling is also described.

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