Using miniature osmotic infusion pumps to maintain tritiated thymidine exposure to cells in culture. | Zendy

J E Neely | Zendy; D. A. Hake | Zendy

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Using miniature osmotic infusion pumps to maintain tritiated thymidine exposure to cells in culture.

Author(s) -

J E Neely,

D. A. Hake

Publication year - 1982

Publication title -

journal of histochemistry and cytochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.971

H-Index - 124

eISSN - 1551-5044

pISSN - 0022-1554

DOI - 10.1177/30.6.7047639

Subject(s) - thymidine , tritiated water , chemistry , tritium , chromatography , cell culture , radiochemistry , tracer , biophysics , biochemistry , biology , in vitro , physics , nuclear physics , genetics

To provide a constant level of tracer doses of tritiated thymidine to cultured cells during continuous infusion, miniature osmotic infusion pumps were used to provide replacement thymidine. By determining the loss of isotope from the media during nonreplacement, the rate of constant infusion replacement to maintain thymidine levels was calculated. The replacement rates were similar for the three cell lines examined and allowed a standard osmotic pump infusion.

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