Use of serial 1-2 micrometer paraffin sections in neuropeptide immunocytochemistry for sequential analysis of different substances contained within the same neurons. | Zendy

U. Schrell | Zendy; Michael V. Sofroniew | Zendy

Open Access

Use of serial 1-2 micrometer paraffin sections in neuropeptide immunocytochemistry for sequential analysis of different substances contained within the same neurons.

Author(s) -

U. Schrell,

Michael V. Sofroniew

Publication year - 1982

Publication title -

journal of histochemistry and cytochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.971

H-Index - 124

eISSN - 1551-5044

pISSN - 0022-1554

DOI - 10.1177/30.6.7047637

Subject(s) - microtome , micrometer , immunocytochemistry , staining , immunohistochemistry , chemistry , anatomy , pathology , biology , physics , medicine , optics

The preparation of serial 1.0, 1.5, or 2.0 micrometer paraffin sections is described. The sections are cut from paraffin blocks with surface areas of up to 5 mm x 10 mm, using glass knives and a Jung Autocut microtome. Large numbers of serial sections can easily be prepared and stained by immunohistochemistry for sequential analysis. These sections are useful for demonstrating several different substances contained within the same neurons or for detailed topographical comparison of neurons that contain different substances within the same nucleus.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research