Ultrastructural immunohistochemical localization of anti-invasion factor (AIF) in bovine cartilage matrix. | Zendy

Frederick H. Wezeman | Zendy; Gwen V. Childs | Zendy

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Ultrastructural immunohistochemical localization of anti-invasion factor (AIF) in bovine cartilage matrix.

Author(s) -

Frederick H. Wezeman,

Gwen V. Childs

Publication year - 1982

Publication title -

journal of histochemistry and cytochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.971

H-Index - 124

eISSN - 1551-5044

pISSN - 0022-1554

DOI - 10.1177/30.6.6178780

Subject(s) - matrix (chemical analysis) , hyaline cartilage , glutaraldehyde , antibody , staining , cartilage , immunohistochemistry , chemistry , antigen , stain , ultrastructure , bovine serum albumin , pathology , microbiology and biotechnology , biology , anatomy , biochemistry , articular cartilage , immunology , osteoarthritis , chromatography , medicine , alternative medicine

Rabbit antibodies prepared against bovine cartilage anti-invasion factor (AIF) were tested for their affinity toward antigenic sites in glutaraldehyde-fixed bovine hyaline cartilage matrix. Ultrastructural localization of the antigen-antibody complex was accomplished by the unlabeled antibody peroxidase-antiperoxidase staining technique. Unextracted and salt-extracted (1 M NaCl or 3 M GuHCl) cartilage slices were incubated with anti-AIF antibodies at a working dilution of 1:20,000. Staining occurred in unextracted matrix distributed throughout the tissue, but with regional variation in the lacunar matrix. Significantly less stain was noted in extracted tissues. The results suggest that at least certain protein components in AIF are morphologically associated with matrix complexes in aldehyde-fixed tissue.

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