The kinetics and homogeneity of endocytosis of a receptor-bound ligand in a heterogeneous cell population studied by flow cytofluorometry. | Zendy

P. Métézeau | Zendy; Lisa DjavadiOhaniance | Zendy; M.E. Goldberg | Zendy

Open Access

The kinetics and homogeneity of endocytosis of a receptor-bound ligand in a heterogeneous cell population studied by flow cytofluorometry.

Author(s) -

P. Métézeau,

Lisa DjavadiOhaniance,

M.E. Goldberg

Publication year - 1982

Publication title -

journal of histochemistry and cytochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.971

H-Index - 124

eISSN - 1551-5044

pISSN - 0022-1554

DOI - 10.1177/30.4.7061830

Subject(s) - endocytosis , internalization , population , ligand (biochemistry) , receptor , kinetics , receptor mediated endocytosis , cell , chemistry , antibody , biophysics , receptor–ligand kinetics , microbiology and biotechnology , biology , biochemistry , immunology , medicine , physics , environmental health , quantum mechanics

A method is described to study quantitatively and rapidly the kinetics of endocytosis of a receptor-bound ligand by cells of various subclasses within a heterogeneous cell population. The time course of the internalization of the bound ligand is followed by labeling, with a fluorescent antibody, the ligand molecules exposed on the cell surface at various times of the endocytosis process, and by measuring with a flow cytofluorometer the fluorescence of a large number of individual cell within the total cell population. A convenient mathematical treatment of the data is proposed for analyzing the experimental results. This method is applied to the kinetic study of the endocytosis of rabbit antibodies (anti-mouse immunoglobulin) by B-lymphocytes within a mouse spleen cell suspension.

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