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Identification of carbonic anhydrase in chick growth-plate cartilage.
Author(s) -
Carol V. Gay,
Richard E. Anderson,
H Schraer,
David S. Howell
Publication year - 1982
Publication title -
journal of histochemistry and cytochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.971
H-Index - 124
eISSN - 1551-5044
pISSN - 0022-1554
DOI - 10.1177/30.4.6801112
Subject(s) - cartilage , epiphyseal plate , immunocytochemistry , carbonic anhydrase , articular cartilage , bone matrix , anatomy , matrix (chemical analysis) , chemistry , enzyme , biology , endocrinology , biochemistry , pathology , medicine , osteoarthritis , alternative medicine , chromatography
Carbonic anhydrase has been localized by immunocytochemistry in the cells and territorial matrix of the hypertrophic and calcifying zones of chick growth-plate cartilage. Adjacent epiphyseal and articular cartilage were not stained. By biochemical assay the activity levels were 61.3, 1.8, 34.7, and 703.3 units/g wet weight for growth plate, epiphyseal/articular cartilage, spongiosa, and blood, respectively. The role of the enzyme in growth plate is discussed.

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