A modified glyoxylic acid-formaldehyde technique for histofluorescence of catecholamine-containing neurons in cryostat sections of the insect brain. | Zendy

Nikolai Klemm | Zendy

Open Access

A modified glyoxylic acid-formaldehyde technique for histofluorescence of catecholamine-containing neurons in cryostat sections of the insect brain.

Author(s) -

Nikolai Klemm

Publication year - 1982

Publication title -

journal of histochemistry and cytochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.971

H-Index - 124

eISSN - 1551-5044

pISSN - 0022-1554

DOI - 10.1177/30.4.6174569

Subject(s) - glyoxylic acid , formaldehyde , catecholamine , chemistry , cryostat , yield (engineering) , fluorophore , biophysics , chromatography , fluorescence , materials science , biochemistry , biology , neuroscience , physics , superconductivity , quantum mechanics , metallurgy

A simple and rapid method is described to demonstrate catecholamine-containing neurons in the insect brain by cryostat sectioning and immersion in an ice-cold SPG (sucrose-potassium phosphate-glyoxylic acid) solution. The fluorescent yield achieved can be further intensified by heating the preparations with formaldehyde vapors. The advantages of the method as compared to the original Falck-Hillarp method are speed, simplicity, and a very distinct photostabile fluorophore. The preparations can be stored for several weeks. The drawback is a lower sensitivity than the Falck-Hillarp method provides.

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