High-grade intensification of the end-product of the diaminobenzidine reaction for peroxidase histochemistry.
Author(s) -
Ferenc Gallyas,
Tamäs J. Görcs,
István Merchenthaler
Publication year - 1982
Publication title -
journal of histochemistry and cytochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.971
H-Index - 124
eISSN - 1551-5044
pISSN - 0022-1554
DOI - 10.1177/30.2.7061820
Subject(s) - peroxidase , thioglycolic acid , chemistry , distilled water , immunohistochemistry , silver nitrate , nuclear chemistry , product (mathematics) , biochemistry , chromatography , enzyme , biology , mathematics , immunology , geometry
A simple and reliable method is described for the intensification of the end-product of the diaminobenzidine reaction demonstrating peroxidase activity. After completing the diaminobenzidine reaction, the preparations to be intensified are immersed first in thioglycolic acid solution, then in distilled water, and finally in a special physical developer containing silver nitrate.
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