Immunohistochemical identification of mast cells in paraffin- and epon-embedded tissues using platelet factor 41. | Zendy

S.T. Shaw | Zendy; Patrick C. Roche | Zendy; Gottfried Schmer | Zendy; L.K. Macaulay | Zendy

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Immunohistochemical identification of mast cells in paraffin- and epon-embedded tissues using platelet factor 41.

Author(s) -

S.T. Shaw,

Patrick C. Roche,

Gottfried Schmer,

L.K. Macaulay

Publication year - 1982

Publication title -

journal of histochemistry and cytochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.971

H-Index - 124

eISSN - 1551-5044

pISSN - 0022-1554

DOI - 10.1177/30.2.7037939

Subject(s) - heparin , immunoperoxidase , antiserum , mast cell , staining , stain , platelet , immunohistochemistry , platelet factor 4 , chemistry , pathology , microbiology and biotechnology , biology , immunology , antibody , biochemistry , medicine , monoclonal antibody

An immunoperoxidase method has been developed for staining heparin in the granules of mast cells. The method employs human platelet factor 4 (or anti-heparin) and a rabbit antiserum to this polypeptide. Platelet factor 4 binds to mast cell heparin and provides the basis for immunoperoxidase staining using the rabbit antiserum. Preliminary studies of mast cells in various tissues indicate that the stain is quite specific for the content of mast cell granules, presumably heparin and possibly other glycosaminoglycans.

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