Adenylate cyclase localization in unfixed specimens of rat oral mucosa and isolated mitochondria. | Zendy

Albert S. Fine | Zendy; Richard W. Egnor | Zendy; Elizabeth Forrester | Zendy; S. S. Stahl | Zendy

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Adenylate cyclase localization in unfixed specimens of rat oral mucosa and isolated mitochondria.

Author(s) -

Albert S. Fine,

Richard W. Egnor,

Elizabeth Forrester,

S. S. Stahl

Publication year - 1982

Publication title -

journal of histochemistry and cytochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.971

H-Index - 124

eISSN - 1551-5044

pISSN - 0022-1554

DOI - 10.1177/30.11.6292286

Subject(s) - adenylate kinase , cyclase , mitochondrion , incubation , biochemistry , chemistry , fibroblast , microbiology and biotechnology , biology , enzyme , in vitro

The cytochemical localization of adenylate cyclase (E.C.4.6.1.1) was demonstrated in unfixed rat oral mucosal specimens and isolated mitochondrial fractions by use of a modified cytochemical reaction mixture. The adenylate cyclase reaction product was observed in the mitochondrial cristae of intact epithelial and fibroblast cells, and in isolated mitochondria after a 10-min incubation in the reaction mixture. The validity of the cytochemical adenylate cyclase method was supported by biochemical studies.

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