Aluminum phosphate visualisation of acid phosphatase activity: a biochemical and x-ray microanalysis study. | Zendy

J. P. Berry | Zendy; J. Hourdry | Zendy; M. Sternberg | Zendy; Peter R. Galle | Zendy

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Aluminum phosphate visualisation of acid phosphatase activity: a biochemical and x-ray microanalysis study.

Author(s) -

J. P. Berry,

J. Hourdry,

M. Sternberg,

Peter R. Galle

Publication year - 1982

Publication title -

journal of histochemistry and cytochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.971

H-Index - 124

eISSN - 1551-5044

pISSN - 0022-1554

DOI - 10.1177/30.1.7054274

Subject(s) - microanalysis , acid phosphatase , phosphate , phosphatase , chemistry , aluminium , alkaline phosphatase , biochemistry , nuclear chemistry , enzyme , organic chemistry

A new method is described that demonstrates acid phosphatase activity in the cells of the proximal tubules of the rat kidney. The method is based on the formation of an insoluble aluminum phosphate precipitate. Microanalysis was used to demonstrate the presence of intracellular aluminum and determine the quantity present under the probe. Parallel biochemical studies showed that the aluminum precipitate was indeed due to acid phosphatase activity.

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