Histochemical and ultrastructural characterization of subendothelial glycoprotein microfibrils interacting with platelets. | Zendy

Philippe Birembaut | Zendy; Y Legrand | Zendy; J Bariéty | Zendy; R Bretton | Zendy; F Fauvel | Zendy; M F Bélair | Zendy; G Pignaud | Zendy; J Caen | Zendy

Open Access

Histochemical and ultrastructural characterization of subendothelial glycoprotein microfibrils interacting with platelets.

Author(s) -

Philippe Birembaut,

Y Legrand,

J Bariéty,

R Bretton,

F Fauvel,

M F Bélair,

G Pignaud,

J Caen

Publication year - 1982

Publication title -

journal of histochemistry and cytochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.971

H-Index - 124

eISSN - 1551-5044

pISSN - 0022-1554

DOI - 10.1177/30.1.6274953

Subject(s) - collagenase , concanavalin a , chemistry , biochemistry , glycoprotein , platelet , ultrastructure , ruthenium red , microbiology and biotechnology , elastin , biology , in vitro , enzyme , anatomy , calcium , immunology , organic chemistry , genetics

The interaction of human blood platelets with collagenase-treated rabbit subendothelium was studied by histochemical ultrastructural methods and by morphometric semi-quantitative analysis. Aortas were deendothelialized and incubated: 1) with a highly purified bacterial collagenase whose specificity was controlled; and 2) with the same collagenase followed by chymotrypsin. For histochemical studies, tannic acid, ruthenium red, and peroxidase-labeled Ricinus communis and concanavalin A were used. Electron microscopy showed that after digestion of fibrillar collagen by collagenase, adherent and aggregated platelets were observed on Ricinus communis-, concanavalin A-, and ruthenium red-positive glycoprotein microfibrils. After successive incubation with collagenase and chymotrypsin, the microfibrils disappeared. No platelets were observed on the remnant amorphous elastin. Morphometric analysis confirmed the interaction of platelets with collagenase-treated subendothelium. In addition, glycoproteins were extracted from collagenase-treated rabbit aortas using 5 M guanidine. Using an in vitro quantitative test, significant platelet adhesion to these glycoproteins was observed. Our results show an interaction between platelets and noncollagenic glycoprotein microfibrils.

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