Open AccessDouble immunocytochemical labeling applying the protein A-gold technique.
Author(s) -
Moı̈se Bendayan
Publication year - 1982
Publication title -
journal of histochemistry and cytochemistry/the journal of histochemistry and cytochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.971
H-Index - 124
eISSN - 1551-5044
pISSN - 0022-1554
DOI - 10.1177/30.1.6172469
Subject(s) - colloidal gold , antiserum , golgi apparatus , chemistry , frozen section procedure , electron microscope , biophysics , immunocytochemistry , antigen , crystallography , biochemistry , cell , nanoparticle , materials science , biology , nanotechnology , physics , optics , pathology , immunology , medicine , endocrinology
In the present study we report the modifications and the different steps of the protein A-gold (pAg) technique that allow the simultaneous demonstration of two antigenic sites on the same tissue section. The labeling is carried out in the following manner: face A of the tissue section is incubated with an antiserum followed by a pAg complex prepared with large gold particles; face B of the same tissue section is then incubated with a second antiserum followed by a pAg complex prepared with small gold particles. Each of the pAg complexes reveals a different antigenic site on opposite faces of the tissue section. The transparency of the section in the electron beam allows the visualization of the gold particles present on both faces. The double labeling pAg technique was applied for the simultaneous demonstration of two secretory proteins in the same Golgi, condensing vacuoles, and zymogen granules of the rat pancreatic acinar cells.