DISTRIBUTION OF AMYLOPHOSPHORYLASE IN VARIOUS TISSUES OF HUMAN AND MAMMALIAN ORGANS

Phosphorylase forms from glucose-1-phosphate in tissue sections a polysaccharide which agrees best with amylose, amylopectin and a lightly branched glycogen, judging from the reactions with iodine, the periodic acid Schiff method and Best's carmine stain. Phosphorylase is demonstrated most intensely in striated skeletal, glossal and cardiac muscle, in tracheal and skeletal cartilage, in the smooth muscle of large and medium arteries, uterus, tubes, vagina, bladder etc., in liver cells and renal collecting tubules, sweat glands and their ducts, hair sheath cells, stratified squamous epithelia of epidermis, tongue, esophagus, cervix uteri and vagina, in some adrenal cortical cells and in nervous tissue. The iodine reaction technique with activator and primer, is the most suitable for the histochemical demonstration of phosphorylase in animal tissues.