ON THE CHEMISTRY OF THE THIOCHOLINE METHOD OF KOELLE | Zendy

H. Malmgren | Zendy; Bengt Sylvén | Zendy

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ON THE CHEMISTRY OF THE THIOCHOLINE METHOD OF KOELLE

Author(s) -

H. Malmgren,

Bengt Sylvén

Publication year - 1955

Publication title -

journal of histochemistry and cytochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.971

H-Index - 124

eISSN - 1551-5044

pISSN - 0022-1554

DOI - 10.1177/3.6.441

Subject(s) - chemistry , sulfide , sulfate , amorphous solid , ammonium sulfate , chromatography , organic chemistry

In the thiocholine method of Koelle as previously described (17), the enzymatic reaction step leads to the formation of crystalline deposits. These have been identified as Cu·thiocholine sulfate. The subsequent ammonium sulfide treatment aiming at the conversion of Cu·thiocholine to CuS implies that the previous crystals become dissolved and replaced by amorphous precipitates. The environmental and other operating factors in this multi-step method are discussed.

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