COMPARATIVE HISTOCHEMICAL AND QUANTITATIVE STUDY OF ADRENAL AND KIDNEY TISSUE BY TETRAZOLIUM TECHNIQUE | Zendy

Joseph Cascarano | Zendy; Benjamin W. Zweifach | Zendy

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COMPARATIVE HISTOCHEMICAL AND QUANTITATIVE STUDY OF ADRENAL AND KIDNEY TISSUE BY TETRAZOLIUM TECHNIQUE

Author(s) -

Joseph Cascarano,

Benjamin W. Zweifach

Publication year - 1955

Publication title -

journal of histochemistry and cytochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.971

H-Index - 124

eISSN - 1551-5044

pISSN - 0022-1554

DOI - 10.1177/3.5.369

Subject(s) - formazan , tetrazolium chloride , tonicity , chemistry , incubation , kidney , pathology , biochemistry , biology , endocrinology , medicine , ischemia

A study was made of the influence of experimental variables, including electrolyte balance, pH, tonicity, length of incubation and dimensions of tissue slice, on the in vitro reduction of tetrazolium. The most discriminating approach included examination of both the histochemical and quantitative expressions of the deposition of formazan. Some of the limitations of neotetrazolium (NT) and 2,3,5 triphenyl tetrazolium chloride (TTC) are discussed, NT being less suitable for quantitative estimates of reduction values.

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