HISTOCHEMICAL DEMONSTRATION OF ACID INORGANIC POLYPHOSPHATASE | Zendy

George G. Berg | Zendy

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HISTOCHEMICAL DEMONSTRATION OF ACID INORGANIC POLYPHOSPHATASE

Author(s) -

George G. Berg

Publication year - 1955

Publication title -

journal of histochemistry and cytochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.971

H-Index - 124

eISSN - 1551-5044

pISSN - 0022-1554

DOI - 10.1177/3.1.22

Subject(s) - polyphosphate , chemistry , phosphate , nitric acid , salt (chemistry) , alcohol , substrate (aquarium) , precipitation , nuclear chemistry , staining , divalent , inorganic chemistry , divalent metal , fixation (population genetics) , ethanol , inorganic phosphate , cofactor , metal , biochemistry , enzyme , organic chemistry , biology , ecology , physics , genetics , meteorology , gene

A substrate solution for histochemical staining of acid inorganic polyphosphatase is prepared by controlled precipitation at pH 3.9, from ingredients totaling .014 M. lead, .05 M. acetate, .02 M. phosphate in the form of Graham's salt, and sufficient nitric acid to titrate. The final concentration of polyphosphate in solution is estimated at 5 x 10 –4 M. in terms of total phosphate. Inorganic polyphosphatase is stainable after a variety of fixation methods and paraffin embedding, but not after treatment with 70% and 80% ethyl alcohol or with excess substrate. The presence of a divalent metallic cofactor, which can be reversibly removed by water, is indicated.

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