The use of monoclonal antibodies against human chorionic gonadotropin for immunoperoxidase staining of normal placenta, pituitary gland, and pituitary adenomas. | Zendy

Torsten Wahlström | Zendy; UlfHåkan Stenman | Zendy; Christina Lundqvist | Zendy; Pirjo Tanner | Zendy; J P Schröder | Zendy; M. Seppälä | Zendy

Open Access

The use of monoclonal antibodies against human chorionic gonadotropin for immunoperoxidase staining of normal placenta, pituitary gland, and pituitary adenomas.

Author(s) -

Torsten Wahlström,

UlfHåkan Stenman,

Christina Lundqvist,

Pirjo Tanner,

J P Schröder,

M. Seppälä

Publication year - 1981

Publication title -

journal of histochemistry and cytochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.971

H-Index - 124

eISSN - 1551-5044

pISSN - 0022-1554

DOI - 10.1177/29.7.6167612

Subject(s) - immunoperoxidase , monoclonal antibody , human chorionic gonadotropin , syncytiotrophoblast , immunohistochemistry , gonadotropin , staining , placenta , biology , antibody , chromophobe cell , pituitary gland , endocrinology , medicine , microbiology and biotechnology , hormone , fetus , immunology , pregnancy , genetics , clear cell

A monoclonal mouse antibody to human chorionic gonadotropin (hCG) was used in a modified unlabeled antibody enzyme-bridge staining method to demonstrate the localization of hCG in normal human placenta, pituitary gland, and six pituitary chromophobe adenomas. Mouse ascitic fluid containing monoclonal antibody could be diluted up to 1:500,000 for detection of hCG in the syncytiotrophoblast, whereas no staining was observed in the pituitary or adenomas even with high antibody concentrations (dilutions from 1:500 upward). Nonspecific background staining was negligible. These results demonstrate that monoclonal antibodies are suitable for immunohistochemical localization of antigens in tissues.

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