DNA analysis and sorting of viable mouse testis cells. | Zendy

W. M. Grogan | Zendy; W F Farnham | Zendy; J M Sabau | Zendy

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DNA analysis and sorting of viable mouse testis cells.

Author(s) -

W. M. Grogan,

W F Farnham,

J M Sabau

Publication year - 1981

Publication title -

journal of histochemistry and cytochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.971

H-Index - 124

eISSN - 1551-5044

pISSN - 0022-1554

DOI - 10.1177/29.6.7252133

Subject(s) - biology , dna , cell sorting , microbiology and biotechnology , homogeneity (statistics) , andrology , spermatogenesis , ploidy , flow cytometry , genetics , gene , endocrinology , computer science , medicine , machine learning

The dye Hoechst 33342 and a 2-parameter cell sorter have been used to measure DNA content in viable testis cells and to sort pachytene spermatocytes and round spermatids from adult mouse testis to virtually 100% homogeneity. Early diploid spermatogenic cells were enriched to 90%, a 10-fold purification. The capability for viable sorting of most testis cell types to homogeneity in numbers suitable for many biochemical applications is demonstrated.

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