An electron microscopic histochemical and analytical X-ray microprobe study of calcification in Bruch's membrane from human eyes. | Zendy

Wendy Davis | Zendy; Ruth G. Jones | Zendy; Herbert K. Hagler | Zendy

Open Access

An electron microscopic histochemical and analytical X-ray microprobe study of calcification in Bruch's membrane from human eyes.

Author(s) -

Wendy Davis,

Ruth G. Jones,

Herbert K. Hagler

Publication year - 1981

Publication title -

journal of histochemistry and cytochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.971

H-Index - 124

eISSN - 1551-5044

pISSN - 0022-1554

DOI - 10.1177/29.5.7252127

Subject(s) - phosphotungstic acid , bruch's membrane , electron microprobe , calcification , chemistry , electron microscope , transmission electron microscopy , microprobe , formic acid , mineralized tissues , anatomy , pathology , biophysics , analytical chemistry (journal) , mineralogy , materials science , chromatography , biochemistry , biology , optics , retinal , medicine , dentin , physics , retinal pigment epithelium , nanotechnology , catalysis

Transmission electron microscopy, electron microprobe analysis, high temperature microincineration, and electron microscopic histochemical procedures were used to study the electron-dense deposits characteristic of the macular aspect of aged human eyes. These inorganic deposits were rich in calcium and phosphorus and selectively removed by flotation on formic acid. The amorphous decalcified masses showed a significant sulfur peak and were readily stained with acidic phosphotungstic acid. The latter observations are indicative of the presence of organic matrical proteoglycan. Such data may be a further indication that proteoglycans are retained at sites of calcification.

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