A novel immunocytochemical model system for specificity and sensitivity screening of antisera against multiple antigens. | Zendy

Lars Larsson | Zendy

Open Access

A novel immunocytochemical model system for specificity and sensitivity screening of antisera against multiple antigens.

Author(s) -

Lars Larsson

Publication year - 1981

Publication title -

journal of histochemistry and cytochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.971

H-Index - 124

eISSN - 1551-5044

pISSN - 0022-1554

DOI - 10.1177/29.3.7016977

Subject(s) - antiserum , antigen , antibody , peptide , sensitivity (control systems) , microbiology and biotechnology , biology , chemistry , immunology , biochemistry , engineering , electronic engineering

A novel, convenient immunocytochemical model system that allows simultaneous testing of multiple peptides and peptide fragments is presented. Screening of antisera for specificity and sensitivity and ready detection of multiple antibody subpopulations is possible with this system. Using the peroxidase-antiperoxidase method, a threshold sensitivity of 100 pg of peptide was attained with the best antisera available. Routine screenings of antisera employ amounts of peptides corresponding to 1.8--180 pmol--concentrations about a thousandfold lower than those used for absorption controls. Because of the minute amounts of antigens needed, specificity screening against rare or costly antigens now becomes feasible.

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