A simple DNA-dependent fluorescence enhancement assay for cell number. | Zendy

C J Adams | Zendy; Brian Storrie | Zendy

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A simple DNA-dependent fluorescence enhancement assay for cell number.

Author(s) -

C J Adams,

Brian Storrie

Publication year - 1981

Publication title -

journal of histochemistry and cytochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.971

H-Index - 124

eISSN - 1551-5044

pISSN - 0022-1554

DOI - 10.1177/29.2.7252122

Subject(s) - fluorometer , fluorescence , dna , cell culture , microbiology and biotechnology , chemistry , cell , chromatography , biochemistry , biology , physics , genetics , quantum mechanics

A DNA-dependent fluorescence enhancement assay using the dye Hoechst 33258 was developed to quantitate cell number in crude detergent lysates of cells. The assay, which uses a simple filter fluorometer, is sensitive enough to measure 1 x 10(4) cells without interference from mercaptans, culture media, and protein solutions. They assay can be done with freeze-thawed lysates. Fluorescence intensity values are linearly related to cell number in the range of 1 x 10(4) to 2.5 x 10(5) cells for L-929, BHK, and CHO-Kl cell lines.

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