Suppression of endogenous avidin-binding activity in tissues and its relevance to biotin-avidin detection systems. | Zendy

Gary S. Wood | Zendy; R Warnke | Zendy

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Suppression of endogenous avidin-binding activity in tissues and its relevance to biotin-avidin detection systems.

Author(s) -

Gary S. Wood,

R Warnke

Publication year - 1981

Publication title -

journal of histochemistry and cytochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.971

H-Index - 124

eISSN - 1551-5044

pISSN - 0022-1554

DOI - 10.1177/29.10.7028859

Subject(s) - avidin , biotin , horseradish peroxidase , endogeny , immunohistochemistry , monoclonal antibody , chemistry , microbiology and biotechnology , biochemistry , antibody , biology , enzyme , immunology

As biotin-avidin systems continue to be developed for applications involving single cells, cell suspensions, and especially tissue sections, the need arises for a method of blocking endogenous avidin-binding activity. One such method is described and its proposed mechanism is discussed. Utilizing this method, endogenous avidin-binding activity was detected and suppressed in selected human and murine tissues, thus facilitating the interpretation of specific immunohistochemical staining utilizing hybridoma monoclonal antibodies in a biotin-avidin-horseradish peroxidase detection system.

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