A simple method for the preparation of cell cultures for ultrastructural investigation. | Zendy

H. Kühn | Zendy

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A simple method for the preparation of cell cultures for ultrastructural investigation.

Author(s) -

H. Kühn

Publication year - 1981

Publication title -

journal of histochemistry and cytochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.971

H-Index - 124

eISSN - 1551-5044

pISSN - 0022-1554

DOI - 10.1177/29.1.7009731

Subject(s) - ultrastructure , in situ , simple (philosophy) , dehydration , cell , materials science , cell culture , chemistry , biology , anatomy , biochemistry , organic chemistry , philosophy , epistemology , genetics

A method is described that allows investigation of cultured cells at the light or electron microscopical level without changing the culturing conditions or the in situ situation during dehydration or embedding. Fixed and dehydrated cell culture layers were cut into small pieces and separated from the plastic dish by adding propylene oxide. With this simple method we could obtain orientated sections of selected cell groups.

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