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Immunocytochemical localization of the posterior lobe hormones and of their carrier proteins.
Author(s) -
Frans Vandesande,
Karen Dierickx,
N. Goossens
Publication year - 1980
Publication title -
journal of histochemistry and cytochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.971
H-Index - 124
eISSN - 1551-5044
pISSN - 0022-1554
DOI - 10.1177/28.5.6991593
Subject(s) - staining , neurophysins , hormone , somatostatin , immunocytochemistry , biology , immunohistochemistry , antigen , endocrinology , immunology , genetics
Serial sections of vertebrate hypothalami were stained with the immunocytochemical peroxidase-antiperoxidase method. In addition to the single staining method, our double staining method was used, which enabled us to visualize two tissue antigens in single tissue sections. In both staining methods, differentially adsorbed antineurohypophysial hormone sera, anti-somatostatin serum and anti-bovine neurophysin sera were used. The results confirm the one hormone, one neuron hypothesis.

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