A rapid method for embedding tissues for electron microscopy using 1,4-dioxane and Polybed 812. | Zendy

Thomas P. Shearer | Zendy; Lawrence G. Hunsicker | Zendy

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A rapid method for embedding tissues for electron microscopy using 1,4-dioxane and Polybed 812.

Author(s) -

Thomas P. Shearer,

Lawrence G. Hunsicker

Publication year - 1980

Publication title -

journal of histochemistry and cytochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.971

H-Index - 124

eISSN - 1551-5044

pISSN - 0022-1554

DOI - 10.1177/28.5.6892923

Subject(s) - electron microscope , embedding , microscopy , materials science , chemistry , nanotechnology , biophysics , biomedical engineering , computer science , pathology , biology , physics , artificial intelligence , optics , medicine

A rapid method for embedding tissues for electron microscopy is described. This method, which can be completed within 5 hr, uses 1,4-dioxane as the final dehydrating agent and Polybed 812 as the embedding medium. Satisfactory preservation of cellular structures is consistently achieved with a variety of normal and diseased tissues. This method may be of particular value to diagnostic electron microscopy laboratories where time and simplicity are critical.

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