Embedment in glycol methacrylate at low temperature allows immunofluorescent localization of a labile tissue protein. | Zendy

Jacqueline Carnegie | Zendy; M. E. McCully | Zendy; Hamish Robertson | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

Embedment in glycol methacrylate at low temperature allows immunofluorescent localization of a labile tissue protein.

Author(s) -

Jacqueline Carnegie,

M. E. McCully,

Hamish Robertson

Publication year - 1980

Publication title -

journal of histochemistry and cytochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.971

H-Index - 124

eISSN - 1551-5044

pISSN - 0022-1554

DOI - 10.1177/28.4.6768792

Subject(s) - glutaraldehyde , paraformaldehyde , chemistry , reagent , methacrylate , embedment , fluorescence , cytoplasm , pipette , biochemistry , chromatography , copolymer , materials science , polymer , organic chemistry , physics , quantum mechanics , composite material

The antigenic activity of a labile protein postulated to the hormone ovine chorionic somatomammotropin (OcS) is preserved in tissue fixed in either glutaraldehyde or paraformaldehyde and low temperature-embedded in the water-soluble plastic glycol methacrylate. Immunofluorescence techniques used on 1--2 micrometer thick sections show that this protein is located in the cytoplasm of certain fetal chorionic cells in association with small spherical bodies, which may be lipid. The use of borohydrate reduction or treatment with Schiff's reagent to reduce glutaraldehyde-induced background fluorescence is described.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research