Determination of immunoreactive melatonin in the colon of the rat by immunocytochemistry. | Zendy

William R. Holloway | Zendy; Lee J. Grota | Zendy; Gregory M. Brown | Zendy

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Determination of immunoreactive melatonin in the colon of the rat by immunocytochemistry.

Author(s) -

William R. Holloway,

Lee J. Grota,

Gregory M. Brown

Publication year - 1980

Publication title -

journal of histochemistry and cytochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.971

H-Index - 124

eISSN - 1551-5044

pISSN - 0022-1554

DOI - 10.1177/28.3.6444434

Subject(s) - melatonin , immunocytochemistry , immunofluorescence , chemistry , antibody , incubation , fluorescence , endocrinology , medicine , microbiology and biotechnology , pathology , biology , biochemistry , immunology , physics , quantum mechanics

Melatonin immunofluorescence in the rat colon was measured with a cadmium sulfide photometer in conjunction with an immunohistological double antibody procedure. The method proved to be very reliable with 5-10% intra- and interassay reliabilities. In addition, manipulation of the primary antibody concentration, the incubation time, and the nature of the first antibody produced predicted changes in fluorescence intensity. Treatment with p-chlorophenylalanine led to a marked reduction of melatonin-specific fluorescence.

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