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The demonstration of antibody specificity by a new technique. The gel electrophoresis-derived enzyme-linked immunosorbent assay (GEDELISA) and its application to antibodies specific for feline leukemia virus.
Author(s) -
H. Lutz,
J. Higgins,
N. C. Pedersen,
G. H. Theilen
Publication year - 1979
Publication title -
journal of histochemistry and cytochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.971
H-Index - 124
eISSN - 1551-5044
pISSN - 0022-1554
DOI - 10.1177/27.8.225379
Subject(s) - feline leukemia virus , antibody , microbiology and biotechnology , polyacrylamide gel electrophoresis , gel electrophoresis , enzyme , leukemia , concanavalin a , chemistry , virology , biology , biochemistry , immunology , in vitro
By combining the high resolution of sodium dodecylsulfate polyacrylamide gel electrophoresis with the sensitivity of enzyme-linked immunosorbent assay (ELISA) antibodies specific for different feline leukemia virus components are characterized. Based on the same principle, Concanavalin A binding sites of FeLV components are also detected.

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