Histochemical detection of carbonic anhydrase with diemthylaminonaphthalene-5-sulfonamide. | Zendy

C Pochhammer | Zendy; Peter Dietsch | Zendy; P. Siegmund | Zendy

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Histochemical detection of carbonic anhydrase with diemthylaminonaphthalene-5-sulfonamide.

Author(s) -

C Pochhammer,

Peter Dietsch,

P. Siegmund

Publication year - 1979

Publication title -

journal of histochemistry and cytochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.971

H-Index - 124

eISSN - 1551-5044

pISSN - 0022-1554

DOI - 10.1177/27.7.112177

Subject(s) - carbonic anhydrase , sulfonamide , chemistry , fluorescence , biochemistry , cytoplasm , enzyme , stereochemistry , physics , quantum mechanics

A new specific method for the detection of carbonic anhydrase, EC 4.2.1.1, in tissues is described. The reaction of carbonic anhydrase with dimethylaminonaphthalene-5-sulfonamide (DNSA) forms a highly fluorescent complex. The specificity of the method is proved by the quenching of this fluorescence with ethoxzolamide (6-ethoxybenzothiazole-5-sulfonamide). The difference in the wavelength makes it possible to absorb the fluorescence of the unbound dimethylaminonaphthalene-5-sulfonamide by filters. Kidney, proventriculus, and bone from chicken have been examined. Carbonic anhydrase has been detected in the cytoplasm of the columnar lining cells, proximal tubule cells, and osteoclasts.

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