Barrier filter for fluorescence microscopy of strongly autofluorescent plant tissues. Application to actin cables in Chara. | Zendy

E A Nothnagle | Zendy; W. W. Webb | Zendy

Open Access

Barrier filter for fluorescence microscopy of strongly autofluorescent plant tissues. Application to actin cables in Chara.

Author(s) -

E A Nothnagle,

W. W. Webb

Publication year - 1979

Publication title -

journal of histochemistry and cytochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.971

H-Index - 124

eISSN - 1551-5044

pISSN - 0022-1554

DOI - 10.1177/27.5.90068

Subject(s) - fluorescence , fluorescence microscope , heavy meromyosin , chara , chloride , ferric , microscopy , biophysics , chemistry , fluorescein , actin , botany , biology , biochemistry , inorganic chemistry , optics , physics , organic chemistry

A liquid barrier filter for use in fluorescence microscopy of strongly autofluorescent plant tissues is described. The filter consists of a methanol solution of cupric chloride and ferric chloride and isolates fluorescein fluorescence from the strong red autofluorescence of photosynthetic plant tissues. Subcortical actin cables in the giant alga Chara are being visualized through use of this filter together with heavy meromyosin labeling.

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