Double labeling immunohistochemical technique provides evidence of the specificity of glial cell markers. | Zendy

M. Saïd Ghandour | Zendy; O.K. Langley | Zendy; G. Vincendon | Zendy; G. Gombos | Zendy

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Double labeling immunohistochemical technique provides evidence of the specificity of glial cell markers.

Author(s) -

M. Saïd Ghandour,

O.K. Langley,

G. Vincendon,

G. Gombos

Publication year - 1979

Publication title -

journal of histochemistry and cytochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.971

H-Index - 124

eISSN - 1551-5044

pISSN - 0022-1554

DOI - 10.1177/27.12.118210

Subject(s) - immunoperoxidase , glial fibrillary acidic protein , immunohistochemistry , carbonic anhydrase , gfap stain , pathology , carbonic anhydrase ii , biology , microbiology and biotechnology , immunocytochemistry , neuroglia , chemistry , biochemistry , enzyme , antibody , central nervous system , monoclonal antibody , medicine , immunology , neuroscience

Sequential treatment of rat cerebellar slices with two antisera, each specific for a different glial cell marker, revealed, by indirect immunofluroescence combined with the immunoperoxidase method on the same tissue section, that carbonic anhydrase isoenzyme II (CA II) is exclusively localized in oligodendrocytes while the glial fibrillary acidic protein (GFA) is, as well established, a specific marker for astrocytes.

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