Open AccessCytochemistry of human catalase. The demonstration of hepatic and renal peroxisomes by a high temperature procedure.
Author(s) -
Frank Roels,
Sidney Goldfischer
Publication year - 1979
Publication title -
journal of histochemistry and cytochemistry/the journal of histochemistry and cytochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.971
H-Index - 124
eISSN - 1551-5044
pISSN - 0022-1554
DOI - 10.1177/27.11.92501
Subject(s) - organelle , cytochemistry , peroxisome , staining , microbody , catalase , ultrastructure , peroxidase , electron microscope , biology , biochemistry , chemistry , pathology , enzyme , microbiology and biotechnology , anatomy , medicine , genetics , physics , optics , gene
The cytochemical demonstration of marker enzymes for subcellular organelles permits light microscopic analysis of their structure and function in normal and diseased tissues. Currently available staining procedures for the peroxidatic activity of catalase in peroxisomes of plant and animal cells yield weak and inconsistent light microscopic staining when applied to human tissues. We have developed a simple and sensitive high temperature procedure that clearly and reproducibly stains these abundant, but poorly understood, organelles in biopsy specimens of human liver and kidney. This method utilizes formaldehyde fixation, a modified diaminobenzidine (DAB) medium, incubation at 45 degrees C and postosmication for both light and electron microscopy.