Scintillation fluid shortens exposure times in autoradiography. | Zendy

B M Stanulis | Zendy; Sue Sheldon | Zendy; Gabriela Lladó Grove | Zendy; V J Cristofalo | Zendy

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Scintillation fluid shortens exposure times in autoradiography.

Author(s) -

B M Stanulis,

Sue Sheldon,

Gabriela Lladó Grove,

V J Cristofalo

Publication year - 1979

Publication title -

journal of histochemistry and cytochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.971

H-Index - 124

eISSN - 1551-5044

pISSN - 0022-1554

DOI - 10.1177/27.10.512316

Subject(s) - scintillator , giemsa stain , chemistry , radiochemistry , tritium , scintillation , microbiology and biotechnology , chromatography , biology , optics , physics , genetics , detector , nuclear physics

Premixed, commercially available scintillation fluids were used to reduce exposure time of tritium (3H) and iodine-125 (125I)-labeled whole cells, and of 3H-labeled Giemsa-banded chromosome preparations. Emulsion-coated slides were dipped into scintillator for no longer than 2 min and exposed in the dark at 4 degrees C. Maximal values for percentage of human diploid cell (WI-38) nuclei labeled with 3H-thymidine of moderate specific activity were obtained in 12 hr. Without scintillator the exposure time was 4 days. Exposure time for cells labeled with 125I-serum was reduced from over 90 days to 14 days. The shortened exposure time for banded chromosomes permitted successful prestaining with Giemsa, a sequence that is not possible without scintillator.

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