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A method for enumeration ot T and B lymphocytes in tissues.
Author(s) -
Frank O. Bastian,
P R Middleditch,
Mattie I. Bossart
Publication year - 1979
Publication title -
journal of histochemistry and cytochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.971
H-Index - 124
eISSN - 1551-5044
pISSN - 0022-1554
DOI - 10.1177/27.10.390034
Subject(s) - enumeration , sonication , lymphocyte , identification (biology) , biology , t lymphocyte , computational biology , pathology , microbiology and biotechnology , immunology , chemistry , chromatography , medicine , mathematics , antigen , combinatorics , botany
A method for the identification of T and B lymphocytes in tissue specimens is described. A sonication technique results in viable relatively pure lymphocyte populations which are easily classified by their surface markers. This readily reproducible method can become a standard laboratory procedure in the evaluation of disease states which require such information related to the classification of lymphocyte cell origin.

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