The preparation of cervical scrape material for automated cytology using gallocyanin chrome-alum stain. | Zendy

P. Eason | Zendy; James H. R. Tucker | Zendy

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The preparation of cervical scrape material for automated cytology using gallocyanin chrome-alum stain.

Author(s) -

P. Eason,

James H. R. Tucker

Publication year - 1979

Publication title -

journal of histochemistry and cytochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.971

H-Index - 124

eISSN - 1551-5044

pISSN - 0022-1554

DOI - 10.1177/27.1.86562

Subject(s) - papanicolaou stain , stain , staining , alum , chemistry , suspension (topology) , materials science , biomedical engineering , pathology , cervical cancer , biology , medicine , metallurgy , genetics , mathematics , homotopy , pure mathematics , cancer

A method is described for preparing cervical scrape specimens for automated analysis on the Cerviscan prescreening system. In order to reduce the cellular clumping found in cervical scrape material, cells are collected in suspension, syringed to disaggregate the cell clumps, and then pipetted onto a glass to give a monolayer of cells. The cells are then stained with gallocyanin chrome-alum to give the required quantitation of nucleic acid content, using a rapid staining procedure. Experimental results are given which show that specimens prepared by this method are more suitable for automated analysis than the conventional Papanicolaou stained preparation.

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