Mass action and acridine orange staining: static and flow cytofluorometry. | Zendy

Claudio Nicolini | Zendy; Andrew S. Belmont | Zendy; Silvio Parodi | Zendy; Stuart R. Lessin | Zendy; Samuel R. Abraham | Zendy

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Mass action and acridine orange staining: static and flow cytofluorometry.

Author(s) -

Claudio Nicolini,

Andrew S. Belmont,

Silvio Parodi,

Stuart R. Lessin,

Samuel R. Abraham

Publication year - 1979

Publication title -

journal of histochemistry and cytochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.971

H-Index - 124

eISSN - 1551-5044

pISSN - 0022-1554

DOI - 10.1177/27.1.86559

Subject(s) - acridine orange , staining , in vivo , differential staining , flow cytometry , chemistry , in vitro , acridine , biophysics , cell , cytoplasm , fluorescence , fluorescein , orange (colour) , microbiology and biotechnology , biochemistry , biology , organic chemistry , genetics , physics , quantum mechanics , food science

We present results involving an approach to acridine orange staining of intact cells based on basic physicochemical considerations. We show by static microfluorometry of several in vitro and in vivo cell lines that the important parameters for such staining are the molar ratio (Formula: see text), and molar concentration of acridine orange. Differential nuclear DNA and cytoplasmic RNA staining are totally controlled by these two parameters. We show this by a physicochemical model of cell-dye interaction. Finally, we use the method to study the growth parameters of complex in vivo cell populations by automated multiparameter flow microfluorometry. We have explored also, both by static and flow systems, the effect on AO-cell staining of various cell pretreatments such as Triton X-100 and chelating agents.

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